|
KMID : 1189420190320030058
|
|
Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology
2019 Volume.32 No. 3 p.58 ~ p.76
|
|
|
Effects of Daecheonglyong-tang on Atopic Dermatitis Induced by DNCB in Mice
|
|
Kim Ji-Eun
Lee Ho-Chan
Kang Eun-Jeong
Choi Jung-Hwa
Kim Jong-Han
Park Soo-Yeon
Jung Min-Young
|
|
|
Abstract
|
|
|
Objectives : This study was designed to examine the effects of Daecheonglyong-tang(DCL) on atopic dermatitis induced by DNCB in mice
Methods : The Nc/Nga mice were divided into 5 groups, and four groups excluding the normal group were applied by 2,4-dinitrochlorobenzene(DNCB), to cause AD and were orally administered with distilled water(negative control), dexamethasone(positive control), and DCL 200 or 400㎎/㎏ once a day for 4 weeks respectively. The visual changes on skin, changes in skin tissue thickness and eosinophil infiltration were observed. IgE, Histamine, Cytokines, immune cells and the amount of gene expression of filaggrin, VEGF, TGF-β1, EGF were measured.
Results : Dermatitis score showed a gross improvement on all DCL groups, similar to or better than positive control. All DCL groups showed no significant change in the basophils, while neutrophils and eosinophils decreased. In only DCL 400 mg/kg groups, white blood cells and mononuclear cells were decreased and lymphocytes were increased. In particular, neutrophils had similar or better effects than the positive control. In all DCL groups, IgE, Histamine, IL-1β, IL-4, IL-5, IL-6 and TNF-α were decreased and IL-2 was increased. In only DCL 400 mg/kg groups, IL-10 decreased and IFN-γ increased. In particular, IL-1β and IFN-γ showed a similar rate of increase and decrease comparing positive control in DCL 400 mg/kg. TGF-β1 was increased in all DCL groups, filaggrin and VEGF were increased in only DCL 400 mg/kg groups. EGF did not make any changes. Epidermis, dermis thickness and eosinophil infiltration were also decreased in all DCL groups.
Conclusions : By increasing Th1 cytokine and decreasing Th2 cytokine, DCL extracts appear to be effective in controlling immune response imbalances, anti-inflammatory and skin regeneration and are likely to be available as a treatment for AD.
|
|
|
KEYWORD
|
|
|
Nc/Nga mice, 2,4-dinitrochlorobenzene(DNCB), Atopic dermatitis(AD), Daecheonglyong-tang(DCL)
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|